JENIFER ALTMAN AWARDS 2005 - TYRONE HAYES, Ph.D.

Tyrone B. Hayes was born in South Carolina to Romeo and Susie Hayes. From very early on, he had an interest in amphibian development and the influence of environment on development. He pursued that interest formally as undergraduate at Harvard, where he earned his Bachelor’s degree in Biology. His thesis work there focused on the effects of temperature on growth, development and sex differentiation and the role of steroid hormones in woodfrogs. He then attended the University of California, Berkeley and completed his doctoral degree in the department of Integrative Biology where he studied further the role of steroid hormones in growth and development in frogs.

Presently, his research continues to focus on the role of steroid hormones in amphibian development. He conducts both laboratory and field studies in the U.S. and Africa. The two main areas of interest are metamorphosis and sex differentiation, but he is also interested in growth (larval and adult) and hormonal regulation of aggressive behavior. Hayes’ work addresses problems on several levels including ecological, organismal, and molecular questions. His main goal is to synthesize ecological/evolutionary, organismal/physiological, and biochemical/molecular studies to learn how an animal translates changes in its external environment to internal changes, how these internal changes are coordinated, what molecular mechanisms are involved, and in turn, how changes at the molecular level affect an animal's ability to adapt to the changes in its external environment.

Most recently, his studies have been used as models to develop laboratory and field techniques to examine the effects of endocrine disrupting contaminants on amphibian development. Most notably his research on atrazine showed that this widely used herbicide is a potent endocrine disruptor that chemically castrates and feminizes frogs at very low doses. His research now focuses on the effects of pesticides mixtures on larval development and the potential role of pesticides in amphibian declines.

Phone: (510)643-1054 (office)(510)643-6264 (fax)
(510)482-9297(home)
Email: tyrone@socrates.berkeley.edu

Selected Publications

2001 Datuin, Jason P., Ng, Kevin P., T.B. Hayes, and H.A. Bern. Effects of glucocorticoids on cartilage growth and response to IGF-I in the tilapia (Oreochromis mossambicus). Gen. Comp. Endocrinol. 121(3): 289-294.

2001 Hayes, Tyrone B.Hyperolius argus endocrine screen test.Official Gazette of the United States Patent and Trademark Office Patents.Sep. 26, 2000. 1238(4):No Pagination. US 6123918; September 26, 2000, 424-92,USA The Regents of the University of California.

2000 Buchholz, D.R. and T.B. Hayes. Larval period comparison for the spadefoot toads Scaphiopus couchii and Spea multiplicata (Anura: Pelobatidae) Herpetologica. 56(4):455-468.).

2000 Noriega, N. and T. B. Hayes. DDT congener effects on secondary sex coloration in the reed frog Hyperolius argus: A partial evaluation of the Hyperolius argus Estrogen Screen. Comparative Biochemistry and Physiology. Comparative Biochemistry and Physiology B. 126B(2): 231-237.
2000 Hayes, T.B. Endocrine Disruptors and Amphibians. IN: G. Linder, D. Sparling, and C. Bishop. Ecotoxicology in Reptiles and Amphibians. Setac Press.

“What you want and what you say should be the same…Neither future nor past can exist alone.”
-Tao Te Ching, Chapter 2

Selected Links:
http://www.mindfully.org/Pesticide/2003/Syngenta-Tyrone-Hayes31oct03.htm
http://teaching.berkeley.edu/dta02/hayes.html
http://www.nationalgeographic.com/xpeditions/lessons/18/g912/hayes1.html
http://www.exploratorium.edu/frogs/researcher/index.html
http://www.jyi.org/volumes/volume10/issue1/features/lee.html
http://www.mindfully.org/Pesticide/2002/Tyrone-Hayes-Curiosity4nov02.htm
http://www.nationalgeographic.com/emerging/tyroneHayes.html
http://students.berkeley.edu/myberkeley/parents.asp?todo=manyfaces7
http://www.pan-uk.org/RCML2005.htm
http://news.minnesota.publicradio.org/features/2005/02/04_bensonl_atrazineupdate/
http://www.mncenter.org/p.asp?WebPage_ID=24&Profile_ID=436
http://sfweekly.com/issues/2004-06-02/feature.html
http://www.sierraclub.org/sierra/200407/profile.asp
http://www.berkeley.edu/news/chancellor/bim/
http://www.nrdc.org/health/pesticides/natrazine.asp
http://www.ourstolenfuture.org/NewScience/wildlife/frogs/2003/2003-1023hayesetal.htm

CURRICULUM VITAE

Tyrone B. Hayes, PhD
Department of Integrative Biology
University of California at Berkeley
Berkeley, California 94720-3140
(510)643-1054 (off.), (510)643-6264 (fax)
(510)482-9297(home)
tyrone@socrates.berkeley.edu

Personal History
Date and place of birth: July 29, 1967; Columbia, SC, USA

Education
1989-93 University of California, Berkeley, CA, Ph.D. 1993, Integrative Biology. (Dissertation title: The Role of Steroids in Growth and Development in Anuran Amphibians)
1985-89 Harvard University, Cambridge, MA, B.A. 1989, Biology (Thesis title: Sex Determination and Differentiation in the Wood Frog: An Investigation of Genetic and Environmental Mechanisms).

Fellowships and Awards
2004 National Geographic Emerging Explorer Award
2004 President’s Citation Award (American Institute for Biological Sciences)
2004 Class of 45, Endowed Chair (UCB)
2004 NIEHS Symposium Grant
2004 Mitch Kapur Foundation Conference Grant
2003 Mitch Kapur Foundation Grant for Undergraduate Research
2003-05 Park Water Foundation Grant
2002-03 Park Water Foundation Grant for Visiting Scholar
2002-03 National Geographic Society Grant
2003 Rose Foundation Grant
2002 Homeland Foundation Grant
2002 World Wildlife Fund Grant
2001 World Wildlife Fund Grant
2001-02 Alton Jones Foundation Grant
2002 Distinguished Teaching Award (UCB)
2001 Distinguished Mentor Award

1999 International Outstanding Young Investigator Award
1999-00 Biology Faculty Award
1999-02 California/Hawaii Seagrant
1998 Bartholomew Award
1998-99 National Geographic Grant
1998 Supplement to NSF CAREER Grant
1997 Supplement to NSF CAREER Grant
1996-01 NSF CAREER Grant
1995 NSF Starter Grant
1993 NSF Postdoctoral Fellowship
1992 Gaige Fund Award
1991 Aubrey Gorbman Best Student Paper Award (ASZ)
1991 Carl P. Kopford Grant
1991 Mentored Research Fellowship Award
1989-93 Berkeley GMP Fellowship
1989 Summa Cum Laude recognition for thesis work
1988-89 Harvard Fellowship Award

Research Experience and Employment

2003- Professor (Endowed Chair), University of California, Berkeley, CA
2000- Associate Professor, University of California, Berkeley, CA
1998- Senior Scientist, Sokoke Inc. El Cerrito, CA 94530
1997- Consultant, Ecorisk, Inc., Ferndale, WA 98248
1994-00 Assistant Professor, University of California, Berkeley, CA
1994 NIH Adjunct Postdoctoral Fellow, NIH, Bethesda, MD
1993-94 NSF Postdoctoral Fellow, University of California, Berkeley, CA
1992-93 Graduate Student Instructor, University of California, Berkeley, CA
1990-93 Research Associate, University of California, Berkeley, CA
1990- Free Lance Technician and Consultant, Biosystems, Tiburon, CA
1989 Field Assistant, Conservation Agency, Wash., D.C.

Papers (Peer-reviewed)

1992 Hayes, T.B. and P. Licht. Gonadal involvement in size sex dimorphism in the African Bullfrog (Pyxicephalus adspersus). J. Exp. Zool. 264(2):130-135.
1993 Hayes, T.B., R. Chan, and P. Licht. Interactions of temperature and steroids in larval growth, development, and metamorphosis in a toad (Bufo boreas). J. Exp. Zool. 266(3):206-215.
1993 Hayes, T.B. and P. Licht. Metabolism of exogenous steroids by anuran larvae. Gen. Comp. Endocrinol. 91:250-258.
1993 Tsai, P.S., T.B. Hayes, and P. Licht. The role of aromatization in testosterone- induced inhibition in female turtles, Trachemys scripta. Biol. Reprod. 50: 144-151.
1995 Hayes, T.B. Interdependence of corticosterone and thyroid hormones in larval growth and development in the western toad (Bufo boreas). I. Thyroid hormone dependent and independent effects of corticosterone on growth and development. J. Exp. Zool. 271(2): 95-102.
1995 Hayes, T.B. and T.-H. Wu. Interdependence of corticosterone and thyroid hormones in larval growth and development in the western toad (Bufo boreas). II. Regulation of corticosterone and thyroid hormones. J. Exp. Zool. 271(2): 103-111.
1995 Hayes, T.B. and P. Licht. Factors influencing testosterone metabolism by anuran larvae. J. Exp. Zool. 271(2): 112-119.
1995 Hayes, T.B. and T.-H. Wu. The role of corticosterone in anuran metamorphosis and its potential role in stress-induced metamorphosis. In Proceedings of the 17th Conference of the European Society of Comparative Endocrinology. Neth. J. Zool. 45(1-2): 107-109.
1995 Hayes, T.B. and T.N. Gill. Hormonal regulation of skin gland development in toad larvae (Bufo boreas): The role of the thyroid hormones and corticosterone. Gen. Comp. Endocrinol. Gen. Comp. Endocrinol. 99:161-168.
1995 Hayes, T.B. An histological examination of the effects of corticosterone in larvae of the Western Toad, Bufo boreas (Anura: Bufonidae), and the Oriental Fire-bellied Toad, Bombina orientalis (Anura: Discoglossidae). J. Morph. 226:297-307.
1997 Hayes, T.B. Amphibian metamorphosis: An historical perspective. Amer. Zool. 37:121-124.
1997 Hayes, T.B. Steroids as modulators of thyroid hormone activity in amphibian development. Amer. Zool. 37(2):185-195.
1997 Hayes, T.B. Steroid-mimicking environmental contaminants: Their potential role in amphibian declines. pp. 145-150, in: Böhme, W., Bischoff, W. and T. Ziegler (eds.): Herpetologia Bonnensis, Bonn (SEH).
1997 Hayes, T.B., T.H. Wu, and T.N. Gill. Similarities in effects of DDT and corticosterone in anuran larvae: Is DDT a stressor or corticosterone mimic? Environ. Toxicol. and Chem. 16(9): 1948-1953.
1997 Hayes, T.B. Hormonal mechanisms as potential constraints on evolution: Examples from the Anura. Amer. Zool. 37: 482-490.
1998 Hayes, T.B. Sex Determination and primary sex differentiation in amphibians. J. Exp. Zool. 281(5): 373-399
1998 Hayes, T.B. Endocrine disruptors in amphibians: Potential impacts and the usefulness of amphibian screens for detecting endocrine disrupting compounds. Science Journal (Kagaku).68: 557-568. (In Japanese).
1998 Pearson, A. K., Hayes, T. B. and Licht, P. (1998) Immunochemical identification of thyrotropes and gonadotropes in the pars distalis and pars tuberalis of the toad (Bufo boreas) with reference to ontogenetic changes. Gen. Comp. Endocrinol. 111: 83-94.
1998 Hayes, T.B. Hormonal regulation of sex differentiation in amphibians, reptiles, and birds. In: (E. Knobil and J. D. Neill, eds.) Encyclopedia of Reproduction. Vol. 4. pp. 102-109. San Diego: Academic Press.
1998 Licht, P. T.B. Hayes, P. Tsai, G. Cunha, H. Kim, M. Golbus, S. Hayward, M.C. Martin, R.B. Jaffe, and S.E. Glickman. Androgens and masculnization of genitalia in the spotted hyaena (Crocuta crocuta). 1. Urogenital morphology and placental androgen production during fetal life. J. Reprod. Fert. 113:105-116.
1999 Hayes, T.B. and K.P. Menendez. The effect of sex steroids on primary and secondary sex differentiation in the sexually dichromatic reedfrog (Hyperolius argus: Hyperolidae) from the Arabuko Sokoke Forest of Kenya. Gen. Comp. Endocrinol. Gen. Comp. Endocrinol. 115: 188-199.
2000 Hayes, T.B. Comparative Endocrinology: A tool for understanding mechanisms and predicting effects of endocrine mimicking xenobiotics. IN: L.J. Guillette (ed.). Environmental Endocrine Disruptors: An Evolutionary Perspective. Taylor and Francis. NY.
2000 Hayes, T.B. Endocrine Disruptors and Amphibians. IN: G. Linder, D. Sparling, and C. Bishop. Ecotoxicology in Reptiles and Amphibians. Setac Press. (In Press).
2000 Noriega, N. and T. B. Hayes. DDT congener effects on secondary
sex coloration in the reed frog Hyperolius argus: A partial evaluation of
the Hyperolius argus Estrogen Screen. Comparative Biochemistry and
Physiology. Comp. Biochem. Physiol. B. 126B(2): 231-237.
2000 Buchholz, D.R. and Hayes, T.B. Larval period comparison for the spadefoot toads Scaphiopus couchii and Spea multiplicata (Anura: Pelobatidae) Herpetologica. (In press).
2001 Hayes, T.B. Hyperolius argus endocrine screen test. Official Gazette of the United States Patent and Trademark Office Patents.Sep. 26, 2000. 1238(4):No Pagination. US 6123918; September 26, 2000, 424-92,USA.
2002 Buchholz, D. and T.B. Hayes. Evolutionary patterns of diversity in spadefoot toad metamorphosis (anura: Pelobatidae). Copeia. (in press).
2002 Hayes, T.B., A. Collins, M. Lee, M. Mendoza, N. Noriega, A.A. Stuart, and A. Vonk. Hermaphroditic, demasculnied frogs following exposure to the herbicide, atrazine, at ecologically relevant doses. (submitted).
2002 Hayes, T.B, K. Haston, M. Tsui, A. Hoang, C. Haeffele, and A. Vonk. Feminization of male frogs in the wild: Water-borne herbicide threatens amphibian populations in parts of the United States. Nature. 419: 895-896.
2004 Hayes, T.B, K. Haston, M. Tsui, A. Hoang, C. Haeffele, and A. Vonk. Atrazine-induced hermaphroditism at 0.1 ppb in American leopard frogs: Evidence from the laboratory and the wild. Environ. Health. Perspect. 111(4): 1-8.
2004 Hayes, T.B. There is no Denying this: Defusing the confusion about atrazine. Bioscience 54(12): 1138-1149.
2005 Hayes, T.B. Welcome to the revolution: Integrative Biology and assessing the impact of endocrine disruptors on environmental and public health. Integrative and Comparative Biology. 45(2): 321-329.
2005 Buchholz, D. and T.B. Hayes. Evolutionary developmental biology: Accelerated metamorphosis in desert frogs. Evolution and Development. (In press).
2005 Hayes, T.B., A.A Stuart, M. Mendoza, A. Collins, N. Noriega, A. Vonk, G. Johnston, R. Liu, and D. Kpodzo. Characterization of atrazine-induced deformities: Support for the demasculinization/feminization hypothesis. Environ. Health. Perspect. (Submitted).
2005 Hayes, T.B. From silent spring to silent night: The potential role of pesticides in amphibian declines. Environ. Health Perspect. (Submitted).

More than 150 abstracts, 400 invited talks and symposia, more than 250 news articles, and featured in 8 documentaries, including the PBS special “Strange Days on Planet Earth: Troubled Waters”.